Neuropathology Consult Service

Muscle Biopsy and Peripheral Nerve Analysis

FORMS

Procedure for Peripheral Nerve and Muscle Biopsies (pdf) 
Neuropathology Request Form (pdf) 

Fax completed forms to the following fax numbers: (859) 257-6838 and (859) 257-5074.

MUSCLE PROCESSING

Routine processing includes preparation of cryostat sections of snap-frozen tissue stained with:

  • Hematoxylin-eosin
  • Modified Gomori trichrome
  • Periodic acid-Schiff
  • Oil red O
  • NADH-TR
  • ATPase (pH 9.4)

When clinically or pathologically indicated, supplemental histochemical and immunohistochemical stains are available in the evaluation of:

  • Metabolic disorder
  • Inflammatory disorders
  • Muscular dystrophies

We also coordinate referral to appropriate reference laboratories for quantitative enzymatic or protein analysis for pertinent disorders (e.g., glycogen or lipid storage diseases; Duchenne muscular dystrophy).

PERIPHERAL NERVE PROCESSING

Routine processing includes preparation of formalin-fixed, paraffin-embedded sections stained with:

  • hematoxylin-eosin
  • Gomori trichrome
  • luxol-fast-blue (myelin)
  • Garvey (axons)

Plastic-embedded semithin sections (glutaraldehyde-fixed) stained with toluidine blue are also evaluated.

When clinically or pathologically indicated, ancillary histochemical and immunohistochemical stains are available (e.g., inflammatory neuropathies).

ULTRASTRUCTURAL ANALYSIS

Electron-microscopic examination is available to classify the diagnosis on some muscle and nerve specimens (glutaraldehyde-fixed sample required).

RESULTS

Reports are relayed by phone and/or fax to the submitting physician in advance of a mailed report.