Chromosome Analysis - Oncology

Chromosome Analysis, Oncology
May be performed on:

  • Bone Marrow Aspirates
    • Adults: 2 - 3 mL in sodium heparin*
    • Children: 1 - 3 mL in sodium heparin
    • The first or second draw of bone marrow from the patient is preferred for chromosome analysis as the first two draws are more concentrated with blasts than subsoquent draws.
    • *4 - 5 mL in sodium heparin is reuquired for bone marrow specimens with orders of chromosome analysis and multiple myeloma FISH panel (new diagnosis or follow up/progression). At least 2 mL of un-clotted bone marrow specimen is required to perform the CD 138 Cell Sort which isolates plasma cells from the bone marrow and provides an enriched population for FISH testing.
  • Peripheral blood for evaluation of leukemia/lymphoma
    • If a bone marrow aspirate cannot be obtained, 7 - 10 mL peripheral blood in sodium heparin can be substituted for bone marrow if the following criteria are met: peripheral blood WBC is > 15,000 and the blast count exceeds 10%.

For the specimens obtained below, sterile transport media (RPMI) may be obtained from the Cytogenetics Lab during work hours (859-257-3736). After hours it may be obtained from Lab Central Receiving (859-323-5431). 

  • Lymph nodes
    • Minimum 5 mm x 5 mm x 5 mm, preferred 10 mm x 10 mm x 10 mm obtained under sterile conditions. Place the specimen in a sterile container with RPMI media.
  • Tumors
    • Minimum 10 mm x 10 mm x 10 mm obtained under sterile conditions. Place the specimen in a sterile container with RPMI media.

Oncology Requisition Form

No